The Caco-2 cell line was isolated from a human colorectal adenocarcinoma (primary site: rectum). When grown to confluence, Caco-2 cells exhibit characteristic differentiation into intestinal epithelial-like cells. These cells express retinoic acid-binding protein I and retinoic acid-binding protein II, and are positive for keratin.

Cell Name: Human colorectal adenocarcinoma cell line

Cell Synonyms: Caco-2; CaCo-2; CACO-2; Caco 2; CACO 2; CACO2; CaCo2; CaCO2; Caco2; Caco-2/ATCC

Catalogue No.: TCH-C146

Species: Human

Tissue Origin: Colorectum

Disease Characteristics: Colorectal adenocarcinoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H +   20% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G146

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 60-80 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection