CaSki / Human cervical squamous cell carcinoma cell line were established from cells derived from a metastatic lesion in the small intestinal mesentery. It has been reported to contain intact HPV-16 (approximately 600 copies per cell) and HPV-18-related sequences. The cells can produce the hCG β-subunit and tumor-associated antigens.

Cell Name: Human cervical squamous cell carcinoma cell line

Cell Synonyms: CaSki; Ca-Ski; Ca Ski; Caski; CASKI

Catalogue No.: TCH-C145

Species: Human

Tissue Origin: Cervix

Disease Characteristic: Cervical cancer

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G145

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection