The C-33 A cell line is one of a series of cell strains established by N. Auersperg from cervical cancer tissue sections. From the outset, the C-33 A cells exhibited a hypodiploid karyotype and epithelial morphology. Karyotypic instability can be observed upon continuous passaging of C-33 A cells. The C-33 A cells possess the retinoblastoma protein (pRB), but it is of abnormal size. Additionally, C-33 A cells show upregulated expression of p53, which contains a point mutation at codon 273 resulting in an Arg→Cys substitution.

Cell Name: Human cervical adenocarcinoma cell line
Cell Synonyms: C-33A; C33A; C33a; C33-A; C-33-A; C-33A; C33

Catalogue No.: TCH-C143
Species: Human
Tissue Origin: Uterus
Disease Characteristic: Cervical cancer

Morphology:Epithelial-like
Growth Properties: Adherent

Culture Medium: MEM + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G143

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 36-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection