The BxPC-3 cell line does not express the cystic fibrosis transmembrane conductance regulator (CFTR). The CFTR-positive cell line is Capan-1.

Cell Name: Human pancreatic adenocarcinoma cell line

Cell Synonyms: BXPC-3; BxPc-3; BxPC-3; Bx-PC3; BXPC3; BxPC3; BxPc3; Biopsy xenograft of Pancreatic Carcinoma line-3

Catalogue No.: TCH-C142
Species: Human
Tissue Origin: Pancreas
Disease Characteristics: Pancreatic cancer

Morphology:Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G142

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 48-60 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection