The source tissue is a papillary invasive ductal carcinoma, with metastasis present in 3 out of 7 regional lymph nodes. The established cells are pleomorphic, comprising epithelioid components and multinucleated giant cells. Mucin-like substances are secreted into the culture medium.

Cell Name: Human breast ductal carcinoma cell line

Cell Synonyms: BT-549; BT 549; BT.549; BT549

Catalogue No.: TCH-C140
Species: Human
Tissue Origin: Breast
Disease Characteristics: Breast ductal carcinoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS + 10 μg/mL Insulin + 1% P/S
Matching Medium Catalog Number: TCH-G140
Passage Ratio: 1:3-1:5, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection