E. Lasfargues and W.G. Coutinho isolated the BT-474 cell line from a solid invasive ductal carcinoma of the breast.

Cell Name: Human breast ductal carcinoma cell line

Cell Synonyms: BT-474; Bt-474; BT474

Catalogue No.: TCH-C139
Species: Human
Tissue Origin: Breast
Disease Characteristics: Ductal Carcinoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G139

Passage Ratio: 1:2, with medium renewal every 2-3 days
Doubling Time: 100-120 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection