Established from a lymph node metastasis of anaplastic papillary thyroid carcinoma in a 63-year-old female; the cells described in the literature do not produce hormones but are partially positive for thyroglobulin and thyroxine (T4).

Cell Name: Human papillary thyroid carcinoma cell line

Cell Synonyms: BHT101; BHT-101

Catalogue No.: TCH-C137
Species: Human
Tissue Origin: Thyroid
Disease Characteristics: Thyroid Cancer

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 20% FBS+ 1% P/S
Matching Medium Catalog Number: TCM-G137
Passage Ratio: 1:2-1:6, with medium renewal every 2-3 days
Doubling Time: 24 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection