The BEAS-2B cell line was derived from normal human bronchial epithelial cells isolated from the pathological tissue of a non-cancerous individual. These cells were infected and cloned using a hybrid adenovirus 12–SV40 virus. BEAS-2B cells retain the ability to undergo squamous differentiation in response to serum stimulation, and they are used for screening chemical or biological agents that induce or affect differentiation and carcinogenesis. BEAS-2B cells are positive for keratin and SV40 antigen staining.

Cell Name: Immortalized human bronchial epithelial cell line

Cell Synonyms: BEAS-2B; Beas-2B; BEAS 2B; BEAS2B; Beas2B; Bronchial Epithelium transformed with Ad12-SV40 2B

Catalogue No.: TCH-C132
Species: Human
Tissue Origin: Lung / Bronchus
Disease Characteristics: Virus-transformed

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G132
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection