Human pancreatic adenocarcinoma cell line(AsPC-1), derived from a 62-year-old Caucasian female, this cell line serves as a suitable transfection host.
Cell Name: Human pancreatic adenocarcinoma cell line (STR-verified)
Cell Synonyms: AsPC-1; AsPc-1; Aspc-1; ASPC-1; As-PC1; ASPC1; AsPC1; Aspc1; AsPc1
Catalogue No.: TCH-C128
Species: Human
Tissue Origin: Pancreas
Disease Characteristics: Metastatic lesion; ascites adenocarcinoma
Morphology: Epithelial-like
Growth Properties: Adherent
Culture Medium: RPMI-1640 + 10% FBS + 1% P/S
Matching Medium Number: TCH-G128
Passage Ratio: 1:3-1:6, with medium change every 2-3 days
Doubling Time: 48-72 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂; Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Quality Control: Negative for bacteria, fungi, and mycoplasma