This cell line was established in 1986 by Amy Aotaki-Keen from the retinal tissue of a healthy 19-year-old male who died in a car accident. The cell line expresses molecular markers specific to retinal pigment cells, such as cellular retinaldehyde-binding protein and PRE-65.

Cell Name: Human retinal pigment epithelial cell line (STR-authenticated)

Cell Synonyms: ARPE-19; ARPE19; Adult Retinal Pigment Epithelial cell line-19;   NTC-200; NTC200

Catalogue No.: TCH-C127

Species: Human
Tissue Origin: Retina
Disease Characteristics: /
Morphology: Epithelial-like
Growth Properties: Adherent
Culture Medium: DF-12 + 10% FBS + 1% P/S
Matching Medium Number: TCH-G127
Passage Ratio: 1:3-1:6, with medium change every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂; Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Quality Control: Negative for bacteria, fungi, and mycoplasma