The AGS cells were derived from an untreated excised tumor fragment; it has been reported that the plating efficiency of AGS cells is 34%.

Cell Name: Human gastric adenocarcinoma cell line

Cell Synonyms: AGS

Catalogue No.: TCH-C124

Species: Human

Tissue Origin: Stomach

Disease Characteristics: Gastric Cancer

Morphology: Epithelial-like

Growth Properties: Adherent

Culture Medium: Ham's F-12K + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G124

Passage Ratio: 1:4-1:6, with medium renewal every 2-3 days
Doubling Time: 24-32 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection