The human cardiomyocyte cell line AC16 was derived from a female donor and immortalized with SV40.

Cell Name: Human ventricular cardiomyocyte cell line[Human hybrid cardiomyocyte]

Cell Synonyms: AC16

Catalogue No.: TCH-C119

Species: Human

Tissue Origin: Heart
Disease Characteristics: /

Morphology: Fibroblast-like

Growth Properties: Adherent
Culture Medium: DF-12 + 12.5% FBS + 1% GlutaMAX + 1% P/S
Passage Ratio: 1:3-1:6, with medium change every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂; Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Quality Control: Negative for bacteria, fungi, and mycoplasma