AAV-293 is derived from the widely used HEK293 cell line but produces higher viral titers. The HEK293 cell line consists of human embryonic kidney cells that have been transfected with sheared adenovirus type 5 DNA. Like HEK293 cells, AAV-293 cells trans-complement the adenovirus E1 gene and can produce infectious adeno-associated virus (AAV) particles when co-transfected with three AAV helper plasmids (one ITR-containing plasmid, pAAV-RC, and an E1-deleted helper plasmid).

Cell Name: Human embryonic kidney cell line, derivative of HEK 293

Cell Synonyms: AAV293; AAV-293

Catalogue No.: TCH-C118
Species: Human
Tissue Origin: Kidney
Disease Characteristics: —
Morphology: Round

Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G118
Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 20-30 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection