The skin cancer cell line A-431 was derived from an 85-year-old female and is one of a series of cell lines established by D.J. Giard et al. It forms rapidly growing subcutaneous tumors resembling malignant melanoma in anti-thymocyte globulin and serum-treated NIH Swiss mice, and can form colonies on normal fibroblasts and agar. This is a hypertriploid human cell line.

Cell Name: Human epidermoid carcinoma cell line

Cell Synonyms: A-431; A431; A431/P

Catalogue No.: TCH-C115

Species: Human

Tissue Origin: Skin

Disease Characteristic: Epidermoid Carcinoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% Glutamax +1% Sodium Pyruvate + 1% P/S
Matching Medium Catalog Number: TCH-G115

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection