A172 cells can form colonies in semi-solid medium; A172 cells do not form tumors in immunosuppressed mice.

Cell Name: Human glioblastoma cell line

Cell Synonyms: A172
Catalogue No.: TCH-C111
Species: Human
Tissue Origin: Brain
Disease Characteristic: Glioblastoma
Morphology: Fibroblast-like

Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G111

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection