The 93T449 cell line was established from a well-differentiated liposarcoma in the retroperitoneum of a 68-year-old female. The karyotype and molecular characteristics of this liposarcoma indicate the presence of ring and large marker chromosomes, which contain amplifications of MDM2, CDK4, and HMGA2.

Cell Name: Human high differentiated liposarcoma cells(STR authenticated)

Cell Synonyms: 93T449

Catalogue No.: TCH-C109

Species: Human
Tissue Origin: Adipose tissue
Disease Feature: Liposarcoma

Morphology: Fibroblast-like

Growth Characteristics: Adherent growth
Culture System: RPMI-1640 + 10% FBS + 1% P/S
Matching Medium No.: TCH-G109
Passage Ratio: 1:3–1:6; change medium every 2–3 days
Doubling Time: 48–72 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Quality Control: Negative for bacteria, fungi, and mycoplasma