$ 800.00
Human bladder carcinoma cell line(5637)
Cat.No. Size
  • Parameters
  • Detail
  • Cell Info
  • References
Cat.No.
TCH-C104
Size
1x10^6
Price
$800.00

This cell line was isolated in 1974 from the bladder cancer tissue of a 68-year-old male; published articles indicate that it can express several growth factors (e.g., SCF, IL-1, IL-6, G-CSF, GM-CSF, etc.).


Cell Name: Human bladder carcinoma cell line

Cell Synonyms: 5637
Catalogue No.: TCH-C104
Species: Human
Tissue Origin: Bladder
Disease Characteristics:Bladder Cancer

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G104

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection


Precautions: 5637 cells are difficult to digest, so it is necessary to extend the digestion time. Digestion should be continued until the cells contract and become round, and when gently tapping the side of the culture flask, the cells can slide off. At this point, digestion can be terminated. The digestion time for these cells is approximately 10 minutes (using 0.25% trypsin).


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