The 2V6.11 was derived in 2001 from the human embryonic kidney cell line 293 [HEK-293]. The 293 [HEK-293] cells were transfected with the plasmid pVgRXR, which carries a Zeocin resistance marker, to generate the 293pVgRXR cell line. Subsequently, these cells were transfected with the plasmid pEKORF6, which contains the Ad5 E4ORF6 gene encoding the E4 24K protein. The pEKORF6 plasmid was derived from the plasmid pIndHydro, which carries a hygromycin resistance gene and includes SV40 viral sequences. The 2V6.11 was initially isolated as a single clone using a cloning ring from cultures containing hygromycin. The human adenovirus E4 34 kDa protein expressed inducibly in 2V6.11 cells can be detected by Western blotting; the 2V6.11 can be used as a tool for studying the adenoviral E4 oncogene.

Cell Name: Human embryonic kidney cells

Cell Synonyms: 2V6.11
Catalogue No.: TCH-C103
Species: Human
Tissue Origin: Kidney
Disease Characteristics: —

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G103
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection