This cell line is derived from 293tsA1609neo and contains the SV40 large T antigen. It is used for retrovirus production, gene expression, and protein production.

Cell Name: Human Embryonic Kidney cells (STR authenticated)

Cell Synonyms: HEK293T; Hek293T; HEK-293T; HEK 293T; HEK-293-T; HEK 293 T;   293-T; 293 T; 293T; Human Embryonic Kidney 293T; 293tsA1609neo

Catalogue No.: TCH-C101

Species:: Human

Tissue Origin: Embryonic Kidney
Disease Characteristics: /

Morphology: Epithelial-like

Growth Properties: Adherent
Culture Medium: DMEM-H + 10% FBS + 1% P/S
Recommended Medium Code: TCH-G101
Passage Ratio: 1:4-1:8; change medium every 2–3 days
Doubling Time: 24–48 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Quality Testing: Negative for bacteria, fungi, and mycoplasma