22RV1 is a human prostate cancer epithelial cell line derived from xenografts (passaged serially in mice where prostate cancer regressed after castration but recurred following re-grafting of androgen-dependent CWR22 from the original host). This cell line expresses prostate-specific antigen. Dihydrotestosterone slightly stimulates cell growth, and Western blot analysis shows that the lysate reacts immunologically with anti-androgen receptor antibodies. Epidermal growth factor (EGF) stimulates cell growth, whereas transforming growth factor beta-1 (TGFβ-1) does not inhibit it. The cells are tumorigenic in nude mice.
Culture Medium:RPMI-1640 + 10% FBS+ 1% P/S Matching Medium Catalog Number: TCH-G100 Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days Doubling Time: 24-48 h Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201 Quality Control: Negative for bacteria, fungi, and mycoplasma detection
Key Points for Culturing 22RV1 Cells:
These cells are density-dependent; the subculturing ratio should not exceed 1:4.
Subculturing ratio and frequency: When cells reach 80% confluency, subculture at a 1:2 ratio, and they will regrow to 80% in 2–3 days. Subculturing at a 1:3 ratio requires over 3 days to reach 80% confluency again.
During cell recovery, centrifugation is required to remove DMSO. After centrifugation, seed the cells in a dish and let them settle for two days, maintaining a stable incubator environment. In the initial post-thawing phase, fewer cells adhere, forming loosely clustered attachments. However, the cells will eventually flatten and spread into a well-formed monolayer, a process that takes 4–5 days.
The cells grow slowly and can only reach up to 90% confluency.