Human peripheral blood B cells originate from the bone marrow and are heterogeneous. The Epstein-Barr virus (EBV) can infect resting (naive) B cells in vitro, utilizing the gene products expressed during its latent phase to alter the B cell life cycle, thereby generating immortalized B lymphoblastoid cell lines (B-LCLs). This has become an effective and commonly used method for B cell immortalization in recent years. B-LCLs are often used as substitutes for peripheral blood B cells and as sources of DNA. Owing to their ability to secrete monoclonal antibodies, function in antigen presentation, and maintain a relatively stable genetic background, they are widely applied across various fields of life sciences.

Human B-lymphoblastoid cell lines (B-LCLs) are immortalized B lymphocyte cell lines cultured in vitro following EBV infection of B cells, capable of unlimited proliferation in culture.

Cell Name: Human B-lymphoblastoid cell lines

Cell Synonyms: B-LCLs
Catalogue No.: ICHX-C005I
Species: Human
Tissue Origin: Peripheral Blood
Disease Characteristics: —
Morphology: Lymphoblastoid-like
Growth Properties: Suspension
Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: ICHX-G005
Passage Ratio: 5×10⁵ - 1×10⁶ cells/mL, with medium renewal every 2-3 days
Doubling Time: 36-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂; Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO; stored in liquid nitrogen
Quality Control: Negative for bacteria, fungi, and mycoplasma