Cell name: HyCyte DB stable cell line for hCXCL9 overexpression

Catalogue No.: CGSC-M34971

Modified Gene: hCXCL9

Gene ID: 4283

Modification Type: Overexpression

Method: Piggy-BAC

Transcript: NM_002416.3

Resistance gene: puro

Fluorescent gene: EGFP

Clone Type: Poolcells

Cellular Identification: PCR, Sequencing

Genus: Homo sapiens

Cell morphology: Lymphocyte-like

Characteristics: Suspension

Culture media: RPMI-1640 supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G424).

Passage ratio: 1:3-1:6, with medium renewal every 2-3 days

Passage period: 48-60h

Culture conditions: 95% air + 5% CO₂ , 37℃

Freezing conditions: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Quality test: bacteria, fungi and mycoplasma negative