HyCyte cell line: HyCyte HT-1080 stable cell line for hFAP overexpression

Catalogue No.: CGSC-M34881

Gene: hC1QBP

Gene ID: 2191

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：2

Target transcript: NM_004460.5

ModificationType: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：HT-1080

Species：Human

Passage：5

Doubling time：24--36h

Growth properties: Adherent

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM)    supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G212).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: DMEM + 30 % FBS + 10 % DMSO; or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hSMARCA4 (-/-)

Quality test: bacteria, fungi and mycoplasma negative