HyCyte cell line: HyCyte HEI-OC1 stable cell line for mSdc1 overexpression

Catalogue No.: CGSC-M34751

Gene: mSdc1

Gene ID: 20969

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：8

Target transcript: NM_011519.2

ModificationType: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：HEI-OC1

Species：Mouse

Passage：5

Doubling time：18--24h

Growth properties: Adherent

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G616).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO_2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: mMiat (-/-)

Quality test: Bacteria, fungi and mycoplasma negative