HyCyte cell line:HyCyte CHO-K1 stable cell line for hTNFRSF10B overexpression

Catalogue No.:CGSC-M34631

Gene: hTNFRSF10B

Gene ID: 8795

Transduction/transfection methods: Lentivirus

Resistance concentration(μg/mL)：10

Target transcript: NM_003842.5

Modification type: Poolcells

Fluorescent gene: copGFP

Resistance gene: Puro

Parental：CHO-K1

Species：Hamster

Passage：5

Doubling time：24-36h

Growth properties: Adherent

Recommended culture media: Ham's F-12K supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin(HyCyte,TCO-G005).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: F-12K + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).