HyCyte cell line: HyCyte MDA-MB-231 stable cell line for hYBX1 overexpression

Catalogue No.: CGSC-M34531

Gene:hYBX1

Gene ID: 4904

Transduction/transfection methods:Transposon

Resistance concentration(μg/mL)：1

Target transcript: NM_004559.5

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：MDA-MB-231

Species：Human

Passage：5

Doubling time：24-36h

Growth properties: Adherent

Recommended culture media: Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G453).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).