HyCyte cell line: HyCyte KYSE-150 stable cell line for hP4HB overexpression

Catalogue No.: CGSC-M34491

Gene: hP4HB

Gene ID: 5034

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：1

Target transcript: NM_000918.4

ModificationType: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：KYSE-150

Species：Human

Passage: 5

Doubling time:18--24h

Growth Properties: Adherent

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS,100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G236).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Genotype: hZNF750 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative

Subculture: Cells are cultured as a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).