HyCyte cell line: HyCyte BALB/3T3 clone A31 stable cell line for mLta overexpression

Catalogue No.: CGSC-M34421

Gene: mLta

Gene ID: 16992

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：4

Target transcript: NM_010735.2

ModificationType: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：BALB/3T3 clone A31

Species：Mouse

Passage：5

Doubling time：24--36h

Growth properties: Adherent

Recommended culture media: Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10%FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G366).

Subculture: Cells are cultured as a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).