HyCyte cell line: HyCyte Hep 3B2.1-7 stable cell line for hNXT1 overexpression

Catalogue No.:CGSC-M34411

Gene: hNXT1

Gene ID: 29107

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：10

Target transcript: NM_013248.3

ModificationType: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：Hep 3B2.1-7

Species：Human

Passage: 5

Doubling time: 24--36h

Growth Properties: Adherent

Recommended culture media:

Minimum Essential Medium (MEM) supplemented with 10 % FBS, 2mM Glutamax,1mM Sodium Pyruvate,1% NEAA,100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G195).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3.

Freezing media: MEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hKLF4 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative