HyCyte cell line: HyCyte U-87MG ATCC stable cell line for hIDH2 overexpression

Catalogue No.: CGSC-M34311

Gene: hIDH2

Gene ID: 3418

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：2

Target transcript: NM_002168.4

Modification type: SingleClone

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：U-87MG ATCC

Species：Human

Passage：5

Doubling time：72-96h

Growth properties: Adherent

Recommended culture media: Minimum Essential Medium (MEM) supplemented with 10% FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G367).

Subculture: Cells are cultured as a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: MEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).