HyCyte cell line: HyCyte T24 stable cell line for hKAT5   overexpression

Catalogue No.: CGSC-M34091

Gene: hKAT5

Gene ID: 10524

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：1

Target transcript: NM_182710.3

ModificationType: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：T24

Species：Human

Passage: 5

Doubling time: 24-48 h

Recommended culture media:

McCoy's 5A Medium supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G352).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: McCoy's 5A + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Quality test: Bacteria, fungi and mycoplasma negative