HyCyte cell line: HyCyte H4-II stable cell line for rInsrr overexpression

Catalogue No.: CGSC-M34041

Gene: rInsrr

Gene ID: 60663

Transduction/transfection methods: Lentivirus

Resistance concentration (μg/mL)：8

Target transcript:NM_022212.2

ModificationType: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：H4-II

Species：Rat

Passage：5

Growth Properties：Adherent

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCR-G630).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: rInsrr (-/-)

Quality test: Bacteria, fungi and mycoplasma negative