HyCyte cell line: HyCyte HEK293 stable cell line for hEIF4E overexpression

Catalogue No.:CGSC-M33881

Gene: hEIF4E

Gene ID: 1977

Transduction/transfection methods: Lentivirus

Resistance concentration(μg/mL)：800

Target transcript: NM_001968.5

Modification type: Poolcells

Fluorescent gene: — —

Resistance gene: Hygro

Parental：HEK293

Species：Human

Passage：5

Doubling time：24-48h

Growth properties: Adherent

Recommended culture media: Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G190).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:8.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).