HyCyte cell line: HyCyte NB4 stable cell line for hBCL6B overexpression

Catalogue No.: CGSC-M33831

Gene: hBCL6B

Gene ID: 255877

Transduction/transfection methods: Lentivirus

Resistance concentration (μg/mL)：0.5

Target transcript: NM_181844.4

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：NB4

Species：Human

Passage：5

Doubling time：18-24h

Growth properties: Suspension

Recommended culture media: RPMI-1640 supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G413).

Subculture:Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. The cell culture density is 3×10⁵ to 5×10⁵ cells per mL.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).