HyCyte cell line: HyCyte NCM460 stable cell line for hGSDMC overexpression

Catalogue No.: CGSC-M33811

Gene: hGSDMC

Gene ID: 56169

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：1

Target transcript: NM_031415.3

Modification type: Poolcells

Fluorescent gene: mCherry

Resistance gene: Puro

Parental：NCM460

Species：Human

Passage：5

Doubling time：18--24h

Growth properties: Adherent

Recommended culture media: RPMI-1640 supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G450).

Subculture:Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:6.3 days. The cell culture density is 3×10⁵ to 5×10⁵ cells per mL.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).