HyCyte cell line: HyCyte Huh-7 stable cell line for hF3 overexpression

Catalogue No.: CGSC-M33711

Gene: hF3

Gene ID: 2152

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：4

Target transcript: NM_001993.5

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：Huh-7

Species：Human

Passage：5

Doubling time：36-48h

Growth properties: Adherent

Recommended culture media: Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 2 mM Glutamax, 1 mM Sodium Pyruvate,100 U/mL Penicilin and 100 µg/mL Streptomycin(HyCyte,TCH-G217).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).