HyCyte cell line: HyCyte NCI-H1299 stable cell line for hSLC16A1 overexpression

Catalogue No.: CGSC-M33621

Gene: hSLC16A1

Gene ID: 6566

Transduction/transfection methods: Lentivirus

Resistance concentration (μg/mL)：4

Target transcript: NM_001166496.2

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：NCI-H1299

Species：Human

Passage：5

Doubling time：24-36h

Growth properties: Adherent

Recommended culture media: RPMI-1640 supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G269).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).