HyCyte cell line: HyCyte MRC-5 stable cell line for hRUNX2 overexpression

Catalogue No.: CGSC-M33611

Gene: hRUNX2

Gene ID: 860

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：2

Target transcript: NM_001024630.4

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：MRC-5

Species：Human

Passage：5

Doubling time：48--60h

Growth properties: Adherent

Recommended culture media: Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G263).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).