HyCyte cell line: HyCyte AML12 stable cell line for mHsp90aa1 overexpression

Catalogue No.: CGSC-M33511

Gene: mHsp90aa1

Gene ID: 15519

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：1

Target transcript: NM_010480.5

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：AML12

Species：Mouse

Passage：5

Doubling time：36-48h

Growth properties: Adherent

Recommended culture media: Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (D/F12) supplemented with 10 % FBS, 1% Insulin-Transferrin-Selenium Supplement, 40 ng/mL Dexamethasone, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCM-G709).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: D/F12 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).