HyCyte cell line: HyCyte MRC-5/TERT-SV40 stable cell line for hP4HA1 overexpression

Catalogue No.: CGSC-M33491

Gene: hP4HA1

Gene ID: 5033

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：400

Target transcript: NM_001142595.2

Modification type: Poolcells

Fluorescent gene: iRFP682

Resistance gene: Neo

Parental：MRC-5

Species：Human

Passage：5

Doubling time：48--60h

Growth properties: Adherent

Recommended culture media: Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G263).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).