HyCyte cell line: HyCyte HCC1937 stable cell line for hBRCA1 overexpression

Catalogue No.: CGSC-M33391

Gene: hBRCA1

Gene ID: 672

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：2

Target transcript: NM_007300.4

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：HCC1937

Species：Human

Passage：5

Doubling time：36-48h

Growth properties: Adherent

Recommended culture media: RPMI-1640 supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin(HyCyte,TCH-G181).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).