HyCyte cell line: HyCyte NALM-6 stable cell line for hZCCHC7 overexpression

Catalogue No.: CGSC-M33381

Gene: hZCCHC7

Gene ID: 84186

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：1

Target transcript: NM_032226.3

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：NALM-6

Species：Human

Passage：5

Doubling time：24-36h

Growth properties: Suspension

Recommended culture media: RPMI-1640 supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G422).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. The cell culture density is 3×10⁵ to 5×10⁵ cells per mL.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).