HyCyte cell line: HyCyte AGS stable cell line for hHKDC1 overexpression

Catalogue No.: CGSC-M33221

Gene: hHKDC1

Gene ID: 80201

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：0.5

Target transcript: NM_025130.4

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：AGS

Species：Human

Passage：5

Doubling time：24-36h

Growth properties: Adherent

Recommended culture media: Ham's F-12K supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin(HyCyte,TCH-G124).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:6.

Freezing media: F-12K + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).