HyCyte cell line: HyCyte CT26 stable cell line for mIgfbp2 overexpression

Catalogue No.: CGSC-M33161

Gene: mIgfbp2

Gene ID: 16008

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：16

Target transcript: NM_008342.4

Modification type: poolcells

Fluorescent gene: luciferase

Resistance gene: Puro

Parental：CT26

Species：Human

Passage：5

Doubling time：24--36h

Growth properties: Adherent

Recommended culture media:RPMI-1640 supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCM-G723).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media:RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).