HyCyte cell line: HyCyte U2OS stable cell line for hPIEZO1 overexpression

Catalogue No.: CGSC-M32881

Gene: hPIEZO1

Gene ID: 9780

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：2

Target transcript: NM_001142864.4，7566 bp]_exon51:c.7529_7546dup：p.Lys2515_Trp2516insSerGluThrMetIleLys

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：U2OS

Species：Human

Passage：5

Doubling time：18-24h

Growth properties: Adherent

Recommended culture media: McCoy's5A Medium supplemented with 10% FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G365).

Subculture: Cells are cultured as a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: McCoy's 5A + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201)