HyCyte cell line: HyCyte NCI-H460 stable cell line for hFAP overexpression

Catalogue No.: CGSC-M32841

Gene: hFAP

Gene ID: 2191

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：1

Target transcript: NM_004460.5

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：NCI-H460

Species：Human

Passage：5

Doubling time：36-48h

Growth properties: Adherent

Recommended culture media: RPMI-1640 supplemented with 10% FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin(HyCyte,TCH-G286).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).