HyCyte cell line: HyCyte PLC/PRF/5 stable cell line for hMFSD2A overexpression

Catalogue No.: CGSC-M32801

Gene: hMFSD2A

Gene ID: 84879

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：2

Target transcript: NM_001136493.3

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：HL-60

Species：Human

Passage：5

Doubling time：35--40h

Growth properties: Adherent

Recommended culture media: Minimum Essential Medium (MEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G298).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3.

Freezing media: MEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).