HyCyte cell line: HyCyte NALM-6 stable cell line for hP4HA2 overexpression

Catalogue No.: CGSC-M32731

Gene: hP4HA2

Gene ID: 8974

Transduction/transfection methods: Lentivirus

Resistance concentration (μg/mL)：1

Target transcript: NM_004199.3

Modification type: Poolcells

Fluorescent gene: Luciferase

Resistance gene: Puro

Parental：NALM-6

Species：Human

Passage：5

Doubling time：24-36h

Growth properties: Suspension

Recommended culture media: RPMI-1640 supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G422).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. The cell culture density is 3×10⁵ to 5×10⁵ cells per mL.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).