HyCyte cell line: HyCyte NIH 3T3 stable cell line for hABCB1 overexpression

Catalogue No.: CGSC-M32681

Gene: hABCB1

Gene ID: 5243

Transduction/transfection methods: Transposon

Resistance concentration(μg/mL)：2

Target transcript: NM_001348945.2

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：NIH3T3

Species：Mouse

Passage：5

Doubling time：24-36h

Growth properties: Adherent

Recommended culture media: Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10% CS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCM-G752).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).