HyCyte cell line: HyCyte HSC-T6 stable cell line for rStat3 overexpression

Catalogue No.: CGSC-M32661

Gene: rStat3

Gene ID: 25125

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：8

Target transcript: NM_012747.3

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：HSC-T6

Species：Rat

Passage：5

Doubling time：18--24h

Growth properties: Adherent

Recommended culture media: Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCR-G627).

Subculture:Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).